Venus Williams quickly followed sister Serena out of Wimbledon as she suffered a 6-2 6-3 defeat at the hands of Tsvetana Pironkova.
Less than an hour after her sibling had been knocked out by Marion Bartoli Venus was comprehensively beaten by the 32nd seed, bringing to an end American interest in the women's draw.
Pironkova again got the better of the five-time champion having also knocked her out last year at the quarter-final stage.
What made the Bulgarian's success on Centre Court even more impressive is that she had won just four matches in 14 tournaments before Wimbledon.
Venus was playing only her second event since withdrawing from the Australian Open with a hip injury and she was well short of her best.
The defeat means that a Williams sister will not be crowned champion at SW19 for the first time since 2006.
Less than an hour after her sibling had been knocked out by Marion Bartoli Venus was comprehensively beaten by the 32nd seed, bringing to an end American interest in the women's draw.
Pironkova again got the better of the five-time champion having also knocked her out last year at the quarter-final stage.
What made the Bulgarian's success on Centre Court even more impressive is that she had won just four matches in 14 tournaments before Wimbledon.
Venus was playing only her second event since withdrawing from the Australian Open with a hip injury and she was well short of her best.
The defeat means that a Williams sister will not be crowned champion at SW19 for the first time since 2006.